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Junko Yano is a leading figure in structural biology and bioinorganic chemistry, renowned for her pioneering work in metalloprotein structure and function, particularly the oxygen-evolving complex of photosystem II. Her major contributions center on advancing X-ray crystallography and spectroscopy techniques to study radiation-sensitive biological samples. Notably, she co-developed high-density crystal grids, enabling efficient data collection from multiple microcrystals—a breakthrough that has garnered over 75 citations and revolutionized high-throughput structural studies at synchrotron and X-ray free-electron laser facilities. This innovation has been critical for femtosecond crystallography, allowing researchers to capture dynamic biological processes without radiation damage. Yano’s impact is profound: her work has shaped our understanding of photosynthetic water splitting, with implications for renewable energy. She has also been instrumental in developing serial femtosecond crystallography methods, earning recognition as a leader in the field. With hundreds of citations across her portfolio, Yano’s research continues to empower structural biologists worldwide, making her a pivotal figure in modern bioinorganic and structural science.