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Sayaka Nagao-Sato is a leading figure in functional genomics, best known for her pivotal contributions to the FANTOM5 project, where she helped pioneer the large-scale mapping of transcription initiation events across the human and mouse genomes. Her work, particularly the landmark 2017 study "FANTOM5 CAGE profiles of human and mouse samples," which has amassed over 360 citations, established a foundational resource for understanding gene regulation at single base-pair resolution. By applying Cap Analysis of Gene Expression (CAGE) coupled with single-molecule sequencing, she enabled the precise monitoring of promoter and enhancer activity across thousands of diverse biological samples. Nagao-Sato also advanced the field methodologically, developing an automated workflow for CAGE library preparation on the HeliScope sequencer, a critical step that improved the reproducibility and scalability of transcriptome analysis. Her research has profoundly impacted our understanding of transcriptional complexity, providing an essential atlas for studies in development, disease, and evolution. Through her technical innovations and large-scale data generation, Nagao-Sato has empowered the global research community to explore the dynamic landscape of gene expression with unprecedented clarity.