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Michiel Krols has made transformative contributions to the field of biological imaging, with a particular focus on advancing three-dimensional scanning electron microscopy (3D SEM) for cellular and tissue-level analysis. His landmark work, "Developing 3D SEM in a broad biological context" (2015, 107 citations), stands as a pivotal resource that contextualized the evolution of electron microscopy from its 1930s origins to modern volumetric imaging techniques. Krols’ research bridges the gap between technological innovation and biological application, enabling researchers to visualize complex cellular architectures—such as neuronal networks and organelle interactions—in unprecedented detail. By systematically outlining the challenges and solutions in 3D SEM, he has empowered the scientific community to explore structural biology at nanoscale resolution, directly impacting fields like neuroscience, cell biology, and pathology. His work is widely cited as a foundational guide for integrating 3D EM into diverse biological contexts, reflecting his role as a key synthesizer and innovator in microscopy. Krols’ contributions continue to shape how scientists perceive the nanoworld, making him a respected figure in the advancement of biological imaging.