Karolina Nekrosiute

Papers

Total Citations

123

H-Index

About

Karolina Nekrosiute is a structural biologist whose work has revolutionized high-throughput crystallography, particularly in the field of fragment-based drug discovery. Her primary research focuses on developing innovative methods for protein crystal soaking and ligand-binding studies, addressing a critical bottleneck in modern structural biology. Nekrosiute’s landmark contribution is the development of a gentle, fast, and effective crystal soaking technique using acoustic droplet ejection, which has transformed how researchers prepare crystals for X-ray diffraction experiments. This method, detailed in her most-cited paper (119 citations), leverages acoustic dispensing to precisely deliver ligands to protein crystals with minimal mechanical stress, dramatically increasing throughput while preserving crystal integrity. By seamlessly integrating with modern synchrotron beamlines—which boast increasing X-ray brightness, robotics, and detector speeds—her approach pushes the sample preparation frontier, enabling high-throughput data collection for ligand-binding studies. This work has been instrumental in accelerating structural biology research, making her a key figure in bridging the gap between advanced beamline capabilities and efficient sample preparation. Her achievements underscore a commitment to solving practical challenges in crystallography, with lasting impact on drug discovery and structural genomics.