Ann Mongan

Papers

Total Citations

328

H-Index

About

Ann Mongan has pioneered the development of high-throughput, multiplexed bioanalytical platforms, with her most influential work centering on the creation of a novel biochip for enzyme-linked immunosorbent assays (ELISAs). Her landmark 1999 paper, "High-Throughput Microarray-Based Enzyme-Linked Immunosorbent Assay (ELISA)," which has garnered over 328 citations, describes a transformative technology that integrates the sensitivity of ELISA with the scalability of microarrays. By engineering an optically flat glass plate with 96 wells defined by a hydrophobic Teflon mask, Mongan enabled simultaneous, parallel analysis of multiple biomarkers—a significant leap from traditional single-analyte methods. This innovation dramatically increased throughput while reducing sample volume and assay time, laying critical groundwork for modern proteomics and clinical diagnostics. Her work is widely recognized for bridging the gap between conventional immunoassays and high-density microarray formats, making it a foundational reference in the field of lab-on-a-chip technologies. Mongan’s contributions have directly influenced the design of next-generation diagnostic tools, and her biochip concept remains a cornerstone for researchers seeking efficient, multiplexed protein detection in both academic and industrial settings.