Nobuhide Doi
Papers
3
Total Citations
58
H-Index
3
About
Nobuhide Doi is a leading figure in bottom-up synthetic biology, whose work focuses on engineering artificial cells to mimic the fundamental spatiotemporal dynamics of life. His research centers on constructing cell-like systems from molecular components to understand and replicate complex biological behaviors, such as reaction-diffusion waves and gene expression patterning. Doi’s major contributions include demonstrating the first regulation of spatiotemporal patterning in artificial cells using a defined protein expression system (2019, 42 citations), a landmark achievement that provides a platform for studying developmental differentiation and homeostasis. He has also pioneered methods to control the periodicity of reaction-diffusion waves in these systems by engineering a two-way energy supplier (2022, 11 citations), offering a tunable approach to dynamic self-organization. Additionally, his development of a novel GUV fusion technique—using calcium and long-chain polyethylene glycol to switch on transcription-translation systems (2021, 5 citations)—addresses a critical bottleneck in synthetic cell construction. Through these innovative contributions, Doi is advancing our ability to build programmable, life-like materials with applications in nanotechnology and cellular biophysics.
Research Focus
Key Achievements
Top Papers
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