Darcy Fyffe

Papers

Total Citations

139

H-Index

About

Darcy Fyffe is a biomedical researcher specializing in 3D cell culture technologies and high-throughput drug screening platforms, with a particular focus on cancer biology and tumor modeling. Fyffe's most significant contribution lies in advancing aqueous two-phase systems (ATPS) as a novel microtechnology for producing tumor spheroids — three-dimensional cellular aggregates that closely mimic the architecture and behavior of avascular tumors in vivo. This work, published in 2014 and accumulating 93 citations, demonstrated how two immiscible polymeric aqueous solutions could be leveraged through microprinting techniques to generate tumor spheroids in a scalable, high-throughput manner, offering a meaningful improvement over traditional 2D cell culture models for anti-cancer drug testing. Building on this foundational research, Fyffe contributed detailed robotic production protocols enabling both mono-culture and co-culture spheroid formation in 96-well plate formats, making the technology more accessible and reproducible for the broader research community. These subsequent publications reflect a commitment to translating innovative laboratory methods into practical, standardized tools. Collectively, Fyffe's work has meaningfully advanced preclinical cancer research by providing more physiologically relevant screening models, supporting researchers worldwide in developing more effective and efficient anti-cancer therapeutics.