Towards Gut Microbiota Sampling Using an Untethered Sampling Device

摘要

Recent studies suggest that human gut microbiota can act as a bio-marker for human health. Also, it can function as a potential tool to understand stress and anxiety. However, the conventional tools have limitations in acquiring a sample of gut microbiota without contamination. In this work, an untethered robotic capsule prototype is developed, which can actively collects the microbiota from the mucosa layer of the small intestine for the first time with a potential to avoid the upstream and downstream contamination. An analytical model for quantifying the peristaltic forces and developing two-way shape memory alloy spring actuator is presented. For the first time, a novel two-way shape memory alloy spring actuator (5 mm x ϕ 4 mm) is used to perform the sampling inside the gut. The spring actuator can apply 675 mN force, which is sufficient to perform in vivo sampling. A specialised experimental setup that can keep the freshly dissected intestine alive for 6 hours is utilised to test the robotic capsule. The robotic capsule prototype has collected an average of 200 μL and 112 μL sample from living porcine duodenal and ileal tissues respectively i.e. in the presence of peristaltic forces. The robotic capsule was also tested on intestine of other species including cow and sheep and collected an average of 160 μL and 185 μL of content respectively from the living postmortem tissues. The collected sample size for all the species is feasible to analyse the microbiota through next generation sequencing techniques. The experimental setup is a reliable proxy to in-vivo behaviour and the robotic capsule experimental result is promising in terms of in situ collection of microbiota.