Natalia Bolotina
Papers
1
Total Citations
35
H-Index
1
About
Natalia Bolotina is a molecular biologist whose work has significantly advanced functional genomics and high-throughput screening technologies. Her primary research focuses on developing innovative tools for gene expression analysis, particularly through the construction of specialized cDNA libraries in yeast systems. Her most impactful contribution, detailed in her 2001 paper "A Human cDNA Expression Library in Yeast Enriched for Open Reading Frames," describes a pioneering high-throughput technique for generating cDNA libraries in *Saccharomyces cerevisiae* that selectively enriches for cloned inserts containing open reading frames (ORFs). This method, which has garnered 35 citations, revolutionized the direct screening of random-primed cDNA libraries by enabling more efficient identification of functional proteins. By constructing a specialized yeast shuttle/expression vector system, Bolotina provided researchers with a powerful platform for studying human gene function in a model organism. Her work has been instrumental in bridging the gap between genomic sequence data and biological function, offering a practical approach for large-scale protein expression and functional analysis. Bolotina’s contributions continue to support advances in molecular biology, particularly in the fields of gene discovery and proteomics.
Research Focus
Key Achievements
Top Papers
- 1A Human cDNA Expression Library in Yeast Enriched for Open Reading Frames35 citations · 2001