Andrew F. Gardner
Papers
2
Total Citations
42
H-Index
2
About
Andrew F. Gardner is a biochemist and molecular biologist whose research bridges the foundational technologies of genomics with cutting-edge enzymology. He is best known for his work on automated DNA sequencing, where his 2011 review on “First Generation” Automated DNA Sequencing Technology (39 citations) provides a definitive account of how the shift from manual to robotic systems revolutionized throughput and cost, laying the groundwork for the Human Genome Project. More recently, Gardner has focused on the mechanistic study of RNA polymerases, developing the HiKER platform (High-throughput Kinetics using capillary Electrophoresis and Robotics). His 2024 paper on this platform reveals how T7, T3, and Sp6 RNA polymerases misincorporate nucleotides, a critical insight given T7 RNAP’s central role in mRNA vaccine production. By replacing traditional denaturing sequencing gels with a high-throughput capillary electrophoresis and robotics system, Gardner has enabled precise, real-time kinetic measurements of polymerase errors. His work not only illuminates fundamental enzyme behavior but also directly impacts the design of more accurate enzymes for therapeutic RNA synthesis.
Research Focus
Key Achievements
Top Papers
- 1“First Generation” Automated DNA Sequencing Technology39 citations · 2011
- 2