Multifunctional optically driven microrobot for realtime 3D bio-manipulation and imaging

Abstract

We have been working on optically driven microrobots capable of single cell manipulation and real-time reaction force sensing in liquid. In this report, we have developed three key technologies to realize 3D manipulation, imaging and sensing of a single living cell. (1) Fluorescent microrobot for long time imaging. (2) Surface functionalization of the robot for effective capture of cells. (3) 3D manipulation and imaging system for realtime observation. These results should lead to integrated micro robotic system that can remotely manipulate living cells in liquid to study cell-cell interactions, cellular response to mechanical stimulation, and to assemble cells into 3D structures for tissue engineering.