Protein purification

One-Step Purification of Recombinant Proteins Using a Nanomolar-Affinity Streptavidin-Binding Peptide, the SBP-Tag

Anthony D. Keefe, David Sloan Wilson, Burckhard Seelig, Jack W. Szostak

Citations: 264 • 2001

Robotic Cloning and Protein Production Platform of the Northeast Structural Genomics Consortium

Thomas Acton, Kristin C. Gunsalus, Rong Xiao, Li Chung, James M. Aramini, Michael Baran, Yiwen Chiang, Teresa Climent, B. Cooper, Natalia G. Denissova, Shawn M. Douglas, J.K. Everett, Chi Kent Ho, Daphne Macapagal, P.K. Rajan, Ritu Shastry, Liang-Yu Shih, G.V.T. Swapna, Michael Wilson, Margaret Wu, Mark Gerstein, Masayori Inouye, J.F. Hunt, G.T. Montelione

Citations: 130 • 2005

An automated method for high-throughput protein purification applied to a comparison of His-tag and GST-tag affinity chromatography

Christoph Scheich, Volker Sievert, Konrad Büssow

Citations: 119 • 2003

Immobilized metal-affinity chromatography protein-recovery screening is predictive of crystallographic structure success

Ryan Choi, Angela Kelley, D.J. Leibly, Stephen N. Hewitt, Alberto J. Napuli, Wesley Van Voorhis

Citations: 103 • 2011

An automated small-scale protein expression and purification screening provides beneficial information for protein production

Henry H. Nguyen, Bruno Martínez-Leo, Natalia Oganesyan, Rosalind Kim

Citations: 43 • 2004

Influence of binding pH and protein solubility on the dynamic binding capacity in hydrophobic interaction chromatography

Pascal Baumann, Kai Baumgartner, Jürgen Hubbuch

Citations: 38 • 2015

A scalable screening of E. coli strains for recombinant protein expression

Luana Galvão Morão, Lívia Regina Manzine, Lívia Oliveira D. Clementino, Carsten Wrenger, Alessandro S. Nascimento

Citations: 25 • 2022

Automation and Optimization of Protein Expression and Purification on a Novel Robotic Platform

Matthew Wollerton, Richard Wales, Jonathan A. Bullock, Ian R. Hudson, Mark Beggs

Citations: 18 • 2006

A fully automated three-step protein purification procedure for up to five samples using the NGC chromatography system

Wolfgang Becker, Anne Scherer, Christine Faust, David Kornblüh Bauer, Simone Scholtes, Ercole Rao, Joachim Hofmann, Rolf Schauder, Thomas Langer

Citations: 18 • 2018

The Center for Optimized Structural Studies (COSS) platform for automation in cloning, expression, and purification of single proteins and protein–protein complexes

Georg Mlynek, Anita Lehner, Jana Neuhold, Sarah Leeb, Július Košťan, Alexej Charnagalov, Peggy Stolt-Bergner, Kristina Djinović‐Carugo, Nikos Pinotsis

Citations: 15 • 2014

Automated Microscale His‐tagged Protein Purification Using Ni‐NTA Magnetic Agarose Beads

Jianxin Meng, Johanna‐Gabriela Walter, Öznur Kökpınar, Frank Stahl, Thomas Scheper

Citations: 13 • 2008

Method to express and purify nm23-H2 protein from baculovirus-infected cells

Livia Garzia, Alessandra Andrè, Angela Amoresano, Anna D’Angelo, R. Martusciello, Claudia Cirulli, Tatsuya Tsurumi, Gennaro Marino, Massimo Zollo

Citations: 11 • 2003

High‐throughput protein purification under denaturating conditions by the use of cation exchange chromatography

Tove Alm, Johanna Stéen, Jenny Ottosson, Sophia Hober

Citations: 10 • 2007

A Rapid and Universal Workflow for Label‐Free‐Quantitation‐Based Proteomic and Phosphoproteomic Studies in Cereals

Mingjie He, Jiahui Wang, Sandra Herold, Lin Xi, Waltraud X. Schulze

Citations: 8 • 2022

Rapid Method to Express and Purify Human Membrane Protein Using the Xenopus Oocyte System for Functional and Low-Resolution Structural Analysis

Benjamin Clémençon, Michael Fine, Philipp Schneider, Matthias A. Hediger

Citations: 8 • 2015

Rapid superparamagnetic‐beads‐based automated immunoseparation of <scp>Z</scp>n‐proteins from <i><scp>S</scp>taphylococcus aureus</i> with nanogram yield

Soňa Křížková, Eva Jílková, Ludmila Krejčová, Natalia Cernei, David Hynek, Branislav Ruttkay-Nedecký, Jiří­ Sochor, Jindřích Kynický, Vojtěch Adam, René Kizek

Citations: 7 • 2012

Robotic High-Throughput Purification of Affinity-Tagged Recombinant Proteins

Simone C. Wiesler, Robert O. J. Weinzierl

Citations: 5 • 2015

Intensification of minor milk protein purification processes

Conan J. Fee, Amita Chand

Citations: 3 • 2006

Protein Precipitation for the Purification of Therapeutic Protein

Christopher W. Morris

Citations: 2 • 2019

A Scalable Screening of <i>E. coli</i> Strains for Recombinant Protein Expression

Luana Galvão Morão, Lívia Regina Manzine, Lívia Oliveira D. Clementino, Carsten Wrenger, Alessandro S. Nascimento

Citations: 2 • 2021