Affinity chromatography

One-Step Purification of Recombinant Proteins Using a Nanomolar-Affinity Streptavidin-Binding Peptide, the SBP-Tag

Anthony D. Keefe, David Sloan Wilson, Burckhard Seelig, Jack W. Szostak

Citations: 264 • 2001

An improved method for the in vitro evolution of aptamers and applications in protein detection and purification

Michael Murphy

Citations: 202 • 2003

Sequential Protein and Peptide Immunoaffinity Capture for Mass Spectrometry-Based Quantification of Total Human β-Nerve Growth Factor

Hendrik Neubert, David Muirhead, Musarat Kabir, Chris Grace, Adriaan Cleton, Rosalin Arends

Citations: 127 • 2012

An automated method for high-throughput protein purification applied to a comparison of His-tag and GST-tag affinity chromatography

Christoph Scheich, Volker Sievert, Konrad Büssow

Citations: 119 • 2003

Immobilized metal-affinity chromatography protein-recovery screening is predictive of crystallographic structure success

Ryan Choi, Angela Kelley, D.J. Leibly, Stephen N. Hewitt, Alberto J. Napuli, Wesley Van Voorhis

Citations: 103 • 2011

High Throughput Production of Recombinant Human Proteins for Crystallography

O. Gileadi, N. Burgess-Brown, S. Colebrook, G. Berridge, P. Savitsky, C. Smee, P. Loppnau, C. Johansson, E. Salah, N. Pantic

Citations: 100 • 2008

An immunoaffinity liquid chromatography–tandem mass spectrometry assay for the quantitation of matrix metalloproteinase 9 in mouse serum

Mireia Fernández Ocaña, Hendrik Neubert

Citations: 69 • 2010

The use of dye—polysaccharide interactions in β-d-glucanase assay

Peter J. Wood

Citations: 44 • 1981

An automated small-scale protein expression and purification screening provides beneficial information for protein production

Henry H. Nguyen, Bruno Martínez-Leo, Natalia Oganesyan, Rosalind Kim

Citations: 43 • 2004

A Collaborative Screening Program for the Discovery of Inhibitors of HCV NS2/3 cis-Cleaving Protease Activity

Mike Whitney, Jeffrey H. Stack, Paul L. Darke, Wei Zheng, Joe Terzo, Jim Inglese, Berta Strulovicil, Lawrence C. Kuo, Brian A. Pollok

Citations: 24 • 2002

Automation and Optimization of Protein Expression and Purification on a Novel Robotic Platform

Matthew Wollerton, Richard Wales, Jonathan A. Bullock, Ian R. Hudson, Mark Beggs

Citations: 18 • 2006

A fully automated three-step protein purification procedure for up to five samples using the NGC chromatography system

Wolfgang Becker, Anne Scherer, Christine Faust, David Kornblüh Bauer, Simone Scholtes, Ercole Rao, Joachim Hofmann, Rolf Schauder, Thomas Langer

Citations: 18 • 2018

Biotinylated-sortase self-cleavage purification (BISOP) method for cell-free produced proteins

Satoko Matsunaga, Kazuhiro Matsuoka, Kouhei Shimizu, Yaeta Endo, Tatsuya Sawasaki

Citations: 16 • 2010

A micro-scale method employing surface plasmon resonance detection for the determination of conditions for immunoaffinity chromatography of proteins

Michael Brigham‐Burke, Daniel J. O’Shannessy

Citations: 16 • 1993

Semi automated production of a set of different recombinant GST-Streptag fusion proteins

Petra Sebastian, Jelena Wallwitz, Štefan Schmidt

Citations: 13 • 2003

Advances in Magnetic Microbead Affinity Selection Screening: Discovery of Natural Ligands to the SARS-CoV-2 Spike Protein

Ruth N. Muchiri, James Kibitel, Margaret A. Redick, Richard B. van Breemen

Citations: 11 • 2021

Boronic acid lectin affinity chromatography (BLAC). 3. Temperature dependence of glycoprotein isolation and enrichment

Marcell Olajos, Ákos Szekrényes, Péter Hajós, Doug T. Gjerde, András Guttman

Citations: 11 • 2010

High‐throughput protein purification under denaturating conditions by the use of cation exchange chromatography

Tove Alm, Johanna Stéen, Jenny Ottosson, Sophia Hober

Citations: 10 • 2007

Industrial Enzymes

Ponnuswamy Vijayaraghavan, S.R.F. Raj, Samuel Gnana Prakash Vincent

Citations: 10 • 2016

IgG-subfractionation by means of hydroxylapatite column chromatography

D. Roelcke, H. Jungfer

Citations: 8 • 1970