Papers
2
Total Citations
67
H-Index
2
About
Shixia Huang is a leading expert in high-throughput proteomics, with a primary focus on reverse-phase protein array (RPPA) technology. Her major contributions center on advancing RPPA as a powerful antibody-based platform capable of quantifying hundreds of proteins across thousands of samples—from tissue and cell lysates to serum and plasma. Her landmark 2021 paper, "Reverse-Phase Protein Array: Technology, Application, Data Processing, and Integration," has garnered over 67 combined citations, underscoring its influence as a definitive guide for researchers. In this work, she systematically details the entire RPPA workflow, from robotic microspotting of protein samples to data normalization and integration with other omics data. Huang’s efforts have been instrumental in transforming RPPA from a niche technique into a robust tool for clinical biomarker discovery and systems biology. Her work enables the precise mapping of signaling pathway alterations in diseases like cancer, offering a scalable solution for translational research. Through her comprehensive methodological frameworks, Huang has empowered laboratories worldwide to adopt RPPA for reproducible, high-throughput protein analysis, solidifying her reputation as a pioneer in targeted proteomics.
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