Sebastian Basso
Papers
1
Total Citations
36
H-Index
1
About
Sebastian Basso is a structural biologist whose work centers on protein crystallization and phase behavior, with a particular focus on lysozyme as a model system. His most cited research, "High-throughput phase-diagram mapping via powder diffraction: a case study of HEWL versus pH" (2005, 36 citations), introduced an innovative high-throughput methodology that combined powder X-ray diffraction with systematic pH variation. By analyzing 44 polycrystalline samples of hen egg-white lysozyme across a pH range of 3.33 to 6.56 at both 277 K and room temperature, Basso demonstrated how powder diffraction can efficiently map protein phase diagrams—a critical step for optimizing crystallization conditions in structural biology. This work provided a rapid, resource-efficient alternative to traditional single-crystal methods, enabling researchers to survey broad parameter spaces with minimal sample consumption. While his citation count reflects focused impact within the crystallization community, the study’s methodological rigor has influenced subsequent high-throughput screening approaches. Basso’s contributions are particularly valuable for students and researchers working on protein structure determination, offering a practical framework for navigating the complex interplay between pH, temperature, and crystal nucleation.
Research Focus
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Top Papers
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