Rainer Pepperkok

Papers

Total Citations

109

H-Index

About

Rainer Pepperkok is a leading figure in cell biology and functional genomics, renowned for pioneering high-content screening microscopy and systematic protein localization studies. His research focuses on developing innovative technologies to unravel the spatial and functional organization of the mammalian cell. A major contribution is his work on siRNA cell arrays for high-content screening, which enabled automated, genome-wide RNA interference studies in living cells—a methodology that has become a cornerstone of modern functional genomics (72 citations). He also played a central role in the German cDNA Consortium, where he led efforts to tag hundreds of novel cDNAs with green fluorescent protein and systematically map their subcellular localizations. This work provided the first large-scale, live-cell classification of protein distribution, bridging functional genomics and proteomics (21 and 16 citations). Pepperkok’s impact lies in his ability to combine cutting-edge imaging, automation, and molecular biology to create tools that empower the broader research community. His achievements have significantly advanced our understanding of cellular architecture and gene function, making him a key contributor to the infrastructure of modern cell biology.