Holger Erfle

European Molecular Biology Laboratory, Heidelberg University

Papers

Total Citations

140

H-Index

About

Holger Erfle is a pioneering biomedical researcher whose work sits at the intersection of genomics, cell biology, and high-throughput screening technologies. Based at Heidelberg University, Erfle has made landmark contributions to the development of innovative cell-based screening platforms that have transformed how scientists study gene function on a genome-wide scale. His most influential work, the development of siRNA cell arrays for high-content screening microscopy (2004, 72 citations), established a robust framework for delivering RNA interference constructs into cultured mammalian cells with unprecedented efficiency and scalability, opening the door to genome-wide functional studies. Building on this foundation, Erfle later pioneered high-density cell arrays designed to dramatically reduce the cost and time burden of large-scale phenotypic screens, advancing preclinical research capabilities. More recently, his team developed an automated 3D light-sheet screening workflow (2020, 40 citations) capable of capturing mitotic phenotypes with remarkable spatiotemporal resolution — a significant leap forward for studying dynamic cellular processes in physiologically relevant 3D cultures. His earlier contributions to DNA sequencing, enabling simultaneous loading of over 200 sample lanes, further demonstrate a career-long dedication to technological innovation that empowers biological discovery at scale.