Mark I. Donnelly
Papers
2
Total Citations
581
H-Index
2
About
Mark I. Donnelly is a structural biologist and protein engineer whose work has significantly advanced high-throughput methods for protein production and characterization. His primary research focuses on developing innovative cloning and expression systems that streamline the study of complex proteins. Donnelly’s most influential contribution is the creation of a novel vector for ligation-independent cloning (LIC) that incorporates a Tobacco Etch Virus (TEV) protease cleavage site, a tool that has been cited over 540 times and remains a cornerstone in recombinant protein purification. This work, published in 2002, enabled researchers to efficiently produce and cleave affinity tags from target proteins, dramatically simplifying structural and functional studies. He further demonstrated the power of these high-throughput protocols by solving the structure of *Bacillus subtilis* ScoAB, a key respiratory protein, using co-expression vectors he designed. His achievements include advancing the efficiency of protein crystallography pipelines and providing foundational tools that have been adopted by laboratories worldwide. Donnelly’s contributions continue to impact fields from enzymology to drug discovery, making him a pivotal figure in modern structural biology.
Research Focus
Key Achievements
Top Papers
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