Jonathan L. Josephs
Papers
2
Total Citations
45
H-Index
2
About
Jonathan L. Josephs is a pharmaceutical scientist whose research centers on drug metabolism, metabolic stability assessment, and the application of high-throughput analytical technologies in drug discovery. His most recognized contribution is the development of the MetFast assay, an automated high-throughput liquid chromatography–mass spectrometry process designed to evaluate the in vitro microsomal cytochrome P450-mediated metabolic stability of drug candidates. Published in 2007, this work has garnered 37 citations and represents a significant advance in pharmaceutical profiling, enabling researchers to rapidly screen compound libraries for first-pass metabolic liabilities early in the drug discovery pipeline. Building on this foundation, Josephs also developed a semi-automated integrated method for simultaneously evaluating compound half-life and identifying metabolic soft spots — the specific structural sites susceptible to enzymatic degradation. Using model compounds such as buspirone, mirtazapine, and verapamil, this work streamlined the characterization of both parent compound disappearance and metabolite formation within a single workflow. Together, Josephs' contributions reflect a sustained commitment to improving the efficiency and depth of early-stage drug candidate profiling, directly supporting more informed decision-making in medicinal chemistry and pharmacokinetic development.
Research Focus
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Top Papers
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