Shin‐ichi Makino

Papers

Total Citations

H-Index

About

Shin-ichi Makino is a structural biologist and biochemist whose research has made significant contributions to the development and automation of cell-free protein synthesis technologies, particularly in the context of structural genomics. Working within the Center for Eukaryotic Structural Genomics (CESG), Makino has been instrumental in advancing wheat cell-free translation systems as powerful platforms for producing proteins that are traditionally difficult to express, including membrane proteins, which remain among the most challenging targets in structural biology. His most impactful work includes pioneering robotic, large-scale applications of cell-free translation for structural studies, demonstrating how automation can dramatically accelerate the pipeline from gene to structure. Complementing this, his contributions to NMR-based high-throughput structure determination established practical workflows for producing stable isotope-labeled eukaryotic proteins — a critical requirement for solution NMR studies. These papers have garnered up to 35 citations, reflecting their value to the structural biology community. Makino's career exemplifies the intersection of biochemical innovation and structural genomics, offering researchers scalable, reproducible tools that continue to shape how complex eukaryotic proteins are studied at the molecular level.