Dominic Esposito
Papers
1
Total Citations
11
H-Index
1
About
Dominic Esposito is a leading figure in high-throughput protein expression and structural biology, best known for pioneering advanced cloning and expression technologies. His key research areas include recombinant protein production, ORF expression systems, and proteomic methods for soluble protein identification. Esposito’s major contribution is the development of Pooled ORF Expression Technology (POET), a groundbreaking approach that combines recombinational cloning with two-dimensional gel electrophoresis and mass spectrometry to rapidly identify open reading frames (ORFs) that yield high levels of soluble, purified protein. This innovation, detailed in his most-cited 2005 paper (11 citations), has significantly streamlined protein production for structural and functional studies, reducing trial-and-error in expression workflows. Beyond POET, Esposito’s work has advanced the efficiency of proteomic pipelines, enabling researchers to tackle complex protein targets with greater speed and reliability. His achievements underscore a commitment to solving practical bottlenecks in molecular biology, making him a key resource for students and researchers seeking robust methods for protein expression and purification.
Research Focus
Key Achievements
Top Papers
- 1Pooled ORF Expression Technology (POET)11 citations · 2005