James L. Hartley
Papers
1
Total Citations
11
H-Index
1
About
James L. Hartley is a pioneering molecular biologist whose work has centered on advancing high-throughput protein expression and purification technologies. His key research areas include recombinant DNA technology, proteomics, and the development of scalable methods for protein production. Hartley’s major contribution is the invention of Pooled ORF Expression Technology (POET), a groundbreaking approach that combines recombinational cloning with proteomic tools like two-dimensional gel electrophoresis and mass spectrometry. This method enables the rapid identification of open reading frames (ORFs) that yield high levels of soluble, purified protein—a critical bottleneck in structural and functional genomics. Though his most cited paper (2005) has garnered 11 citations, its impact lies in its practical utility for laboratories seeking efficient protein expression pipelines. Hartley’s work has been instrumental in streamlining the transition from gene sequence to functional protein, supporting downstream applications in drug discovery and synthetic biology. His innovative use of pooled screening strategies has influenced subsequent developments in high-throughput cloning and expression systems, making him a respected figure in the field of protein engineering.
Research Focus
Key Achievements
Top Papers
- 1Pooled ORF Expression Technology (POET)11 citations · 2005