Interfacing Capillary/Nano LC with MALDI/MS for High-Throughput Proteomics

Abstract

Besides higher efficiency and sensitivity, another advantage of capillary/nano LC is the volume compatibility with the μ-liter fraction requirements of MALDI targets. One of the challenges is the collection of nanoliter volumes without remixing the separated compounds and losing chromatographic separation. In this work, a high-precision X/Y/Z robotic system, ProbotTM, was used that allows for the collection of tiny nanoliter volumes with zero chromatographic dispersion.