Increasing Sample Preparation Throughput Using Monolithic Methacrylate Polymer as Packing Material for 96‐Tip Robotic Device

Abstract

Abstract In this work, a laboratory robot has been used to accomplish a system for high sample cleanup throughput. The robot operating in a 96‐well format was furnished with 96 polypropylene tips packed with a chemically bonded monolithic methacrylate plug as sample adsorbent. Using this system, 96 samples could be handled in 2 minutes. Polypropylene tips were furnished with a chemically bonded monolithic methacrylate plug as sample adsorbent. Roscovitine and lidocaine in plasma samples were used as model substances. The validation of the methodology showed that the accuracy values of quality control samples (QC) were between +15%, and precision had a maximum deviation of 11%. The standard curve was obtained within the concentration range 14‐5600 nM in both plasma and water samples. The regression correlation coefficients (R2) for plasma and water samples were ≥0.999 for all runs.