Mutant

Ultrahigh-throughput screening in drop-based microfluidics for directed evolution

Jeremy J. Agresti, Eugene Antipov, Adam R. Abate, Keunho Ahn, Amy C. Rowat, Jean‐Christophe Baret, Manuel Márquez, Alexander M. Klibanov, Andrew D. Griffiths, David A. Weitz

Citations: 1147 • 2010

A high-throughput gene knockout procedure for <i>Neurospora</i> reveals functions for multiple transcription factors

Hildur V. Colot, Gyungsoon Park, G. Turner, Carol S. Ringelberg, Christopher M. Crew, Liubov Litvinkova, Richard L. Weiss, Katherine A. Borkovich, Jay Dunlap

Citations: 1143 • 2006

A Robot-Based Platform to Measure Multiple Enzyme Activities in Arabidopsis Using a Set of Cycling Assays: Comparison of Changes of Enzyme Activities and Transcript Levels during Diurnal Cycles and in Prolonged Darkness[W]

Yves Gibon, Oliver E. Blaesing, Jan Hannemann, Petronia Carillo, Melanie Höhne, Janneke Hendriks, Natalia Palacios‐Rojas, Joanna Cross, Joachim Selbig, Mark Stitt

Citations: 547 • 2004

Temporal responses of transcripts, enzyme activities and metabolites after adding sucrose to carbon‐deprived Arabidopsis seedlings

Daniel Osuna, Björn Usadel, Rosa Morcuende, Yves Gibon, Oliver E. Bläsing, Melanie Höhne, Manuela Günter, Beate Kamlage, Richard N. Trethewey, Wolf‐Rüdiger Scheible, Mark Stitt

Citations: 300 • 2007

High-throughput, quantitative analyses of genetic interactions in E. coli

Athanasios Typas, Robert J. Nichols, Deborah A. Siegele, Michael Shales, Sean R. Collins, Bentley Lim, Hannes Braberg, Natsuko Yamamoto, Rikiya Takeuchi, Barry L. Wanner, Hirotada Mori, Jonathan S. Weissman, Nevan J. Krogan, Carol A. Gross

Citations: 246 • 2008

A permease‐like protein involved in ER to thylakoid lipid transfer in Arabidopsis

Changcheng Xu

Citations: 215 • 2003

High resolution functional analysis of antibody-antigen interactions

Lei Jin, B M Fendly, James A. Wells

Citations: 213 • 1992

Directed evolution of thymidine kinase for AZT phosphorylation using DNA family shuffling

Fred C. Christians, Léonardo Scapozza, Andreas Crameri, Gerd Folkers, Willem P.C. Stemmer

Citations: 127 • 1999

Advances in directed protein evolution by recursive genetic recombination: applications to therapeutic proteins

Aaron L. Kurtzman, Sridhar Govindarajan, Katherine Vahle, Jennifer T. Jones, Volker Heinrichs, Phillip A. Patten

Citations: 111 • 2001

Intramolecular Isopeptide Bonds Give Thermodynamic and Proteolytic Stability to the Major Pilin Protein of Streptococcus pyogenes

Hae Joo Kang, Edward N. Baker

Citations: 111 • 2009

Insect Herbivory-Elicited GABA Accumulation in Plants is a Wound-Induced, Direct, Systemic, and Jasmonate-Independent Defense Response

Sandra S. Scholz, Michael Reichelt, Dereje W. Mekonnen, Frank Ludewig, Axel Mithöfer

Citations: 109 • 2015

Expression of nitrous oxide reductase in Paracoccus denitrificans is regulated by oxygen and nitric oxide through FnrP and NNR

Linda Bergaust, Rob J. M. van Spanning, Åsa Frostegård, Lars R. Bakken

Citations: 108 • 2011

A personalized platform identifies trametinib plus zoledronate for a patient with KRAS-mutant metastatic colorectal cancer

Erdem Bangi, Celina Ang, Peter Smibert, Andrew Uzilov, Alexander Teague, Yevgeniy Antipin, Rong Chen, Chana Hecht, Nelson Gruszczynski, Wesley J. Yon, Denis Malyshev, Denise Laspina, Isaiah Selkridge, Aye S. Moe, Chun Yee Lau, Patricia Taik, Eric Wilck, Aarti Sonia Bhardwaj, Max W. Sung, Sara Kim, Kendra Yum, Robert Sebra, Michael Donovan, Krzysztof Misiukiewicz, Eric E. Schadt, Marshall R. Posner, Ross Cagan

Citations: 107 • 2019

Large-scale robot-assisted genome shuffling yields industrial Saccharomyces cerevisiae yeasts with increased ethanol tolerance

Tim Snoek, Martina Picca Nicolino, Stefanie Van den Bremt, Stijn F. L. Mertens, Veerle Saels, Alex Verplaetse, Jan Steensels, Kevin J. Verstrepen

Citations: 105 • 2015

Directed Evolution of an Esterase from Pseudomonas fluorescens. Random Mutagenesis by Error-Prone PCR or a Mutator Strain and Identification of Mutants Showing Enhanced Enantioselectivity by a Resorufin-Based Fluorescence Assay

Erik Henke, Uwe T. Bornscheuer

Citations: 103 • 1999

Persistent p53 Mutations in Single Cells from Normal Human Skin

Ling Gao, Åsa Persson, Berit Berne, Mathias Uhlén, Joakim Lundeberg, Fredrik Pontén

Citations: 100 • 2001

The Sulfolipids 2′-<i>O</i>-Acyl-Sulfoquinovosyldiacylglycerol and Sulfoquinovosyldiacylglycerol Are Absent from a<i>Chlamydomonas reinhardtii</i>Mutant Deleted in<i>SQD1</i> 

Wayne R. Riekhof, Michael E. Ruckle, Todd A. Lydic, Barbara B. Sears, Christoph Benning

Citations: 99 • 2003

Highly parallel lab evolution reveals that epistasis can curb the evolution of antibiotic resistance

Marta Lukačišinová, Booshini Fernando, Tobias Bollenbach

Citations: 84 • 2020

Screening the yeast deletant mutant collection for hypersensitivity and hyper‐resistance to sorbate, a weak organic acid food preservative

Mehdi Mollapour, Dahna M. Fong, Nicholas Harris, Suzanne Thompson, Christoph Schüller, Karl Kuchler, Peter W. Piper

Citations: 82 • 2004

Phenotypic analysis of gene deletant strains for sensitivity to oxidative stress

Vincent J. Higgins, Nazif Alic, Geoffrey W. Thorpe, Michael Breitenbach, Veronica Larsson, Ian W. Dawes

Citations: 78 • 2001