Directed evolution

Ultrahigh-throughput screening in drop-based microfluidics for directed evolution

Jeremy J. Agresti, Eugene Antipov, Adam R. Abate, Keunho Ahn, Amy C. Rowat, Jean‐Christophe Baret, Manuel Márquez, Alexander M. Klibanov, Andrew D. Griffiths, David A. Weitz

Citations: 1147 • 2010

Directed evolution of thymidine kinase for AZT phosphorylation using DNA family shuffling

Fred C. Christians, Léonardo Scapozza, Andreas Crameri, Gerd Folkers, Willem P.C. Stemmer

Citations: 127 • 1999

Advances in directed protein evolution by recursive genetic recombination: applications to therapeutic proteins

Aaron L. Kurtzman, Sridhar Govindarajan, Katherine Vahle, Jennifer T. Jones, Volker Heinrichs, Phillip A. Patten

Citations: 111 • 2001

Large-scale robot-assisted genome shuffling yields industrial Saccharomyces cerevisiae yeasts with increased ethanol tolerance

Tim Snoek, Martina Picca Nicolino, Stefanie Van den Bremt, Stijn F. L. Mertens, Veerle Saels, Alex Verplaetse, Jan Steensels, Kevin J. Verstrepen

Citations: 105 • 2015

Directed Evolution of an Esterase from Pseudomonas fluorescens. Random Mutagenesis by Error-Prone PCR or a Mutator Strain and Identification of Mutants Showing Enhanced Enantioselectivity by a Resorufin-Based Fluorescence Assay

Erik Henke, Uwe T. Bornscheuer

Citations: 103 • 1999

Directed evolution of a maltogenic α-amylase from Bacillus sp. TS-25

A. Daniel Jones, Michael Lamsa, Torben P. Frandsen, T. Spendler, Paul Harris, Alan Sloma, Fan Xu, J NIELSEN, J.A. CHERRY

Citations: 64 • 2008

Altering the Substrate Specificity of Cephalosporin Acylase by Directed Evolution of the β-Subunit

Linda G. Otten, Charles F. Sio, Johanna Vrielink, Robbert H. Cool, Wim J. Quax

Citations: 62 • 2002

Protein engineering via Bayesian optimization-guided evolutionary algorithm and robotic experiments

Ruyun Hu, Lihao Fu, Yongcan Chen, Junyu Chen, Yu Qiao, Tong Si

Citations: 60 • 2022

Focused Directed Evolution of Pentaerythritol Tetranitrate Reductase by Using Automated Anaerobic Kinetic Screening of Site‐Saturated Libraries

Martyn Hulley, Helen S. Toogood, Anna Fryszkowska, David Mansell, Gill Stephens, John M. Gardiner, Nigel S. Scrutton

Citations: 58 • 2010

Gel microdroplet–based high-throughput screening for directed evolution of xylanase-producing Pichia pastoris

Chunxuan Ma, Zheng Lin Tan, Ying Lin, Shuangyan Han, Xin‐Hui Xing, Chong Zhang

Citations: 53 • 2019

Human antibody libraries: A race to engineer and explore a larger diversity

Philippe Mondon

Citations: 53 • 2007

New approaches to enzymatic glycoside synthesis through directed evolution

Roman Kittl, Stephen G. Withers

Citations: 52 • 2010

Heterologous expression of Melanocarpus albomyces cellobiohydrolase Cel7B, and random mutagenesis to improve its thermostability

Sanni Voutilainen, Harry Boer, Markus B. Linder, Terhi Puranen, Juha Rouvinen, Jari Vehmaanperä, Anu Koivula

Citations: 45 • 2007

An automated pipeline for the screening of diverse monoterpene synthase libraries

Nicole G. H. Leferink, Mark S. Dunstan, Katherine A. Hollywood, Neil Swainston, Andrew Currin, Adrian J. Jervis, Eriko Takano, Nigel S. Scrutton

Citations: 37 • 2019

Imaging-Based Screening Platform Assists Protein Engineering

Arne Fabritius, David Ng, Andreas M. Kist, Mutlu Erdoğan, Rubén Portugues, Oliver Griesbeck

Citations: 33 • 2018

Directed evolution of a cyclodipeptide synthase with new activities <i>via</i> label-free mass spectrometric screening

Songya Zhang, Jing Zhu, Shuai Fan, Wenhao Xie, Zhaoyong Yang, Tong Si

Citations: 28 • 2022

Automated Purification of His ₆ -Tagged Proteins Allows Exhaustive Screening of Libraries Generated by Random Mutagenesis

Thomas Lanio, Albert Jeltsch, Alfred Pingoud

Citations: 24 • 2000

Efficient screening for new amino acid dehydrogenase activity: Directed evolution of Bacillus sphaericus phenylalanine dehydrogenase towards activity with an unsaturated non-natural amino acid

Sihong Chen, Paul C. Engel

Citations: 19 • 2009

Ultrahigh-Throughput Screening of Single-Cell Lysates for Directed Evolution and Functional Metagenomics

Fabrice Gielen, Pierre-Yves Colin, Philip Mair, Florian Hollfelder

Citations: 19 • 2017

Robotic workflows for automated long-term adaptive laboratory evolution: improving ethanol utilization by Corynebacterium glutamicum

Lars Halle, Niels Hollmann, Niklas Tenhaef, Lea Mbengi, Christiane Glitz, Wolfgang Wiechert, Tino Polen, Meike Baumgart, Michael Bott, Stephan Noack

Citations: 15 • 2023