Uwe T. Bornscheuer
Universitätsmedizin Greifswald, University of Stuttgart, Universität Greifswald
Papers
4
Total Citations
281
H-Index
4
About
Uwe T. Bornscheuer is a distinguished enzymologist and protein engineer whose research has profoundly shaped the fields of directed evolution, high-throughput enzyme screening, and biocatalysis. His work centers on harnessing and optimizing enzyme activity for industrial and synthetic applications, with a particular focus on developing innovative screening methodologies that make enzyme engineering more efficient and accessible. Bornscheuer's landmark 1999 study on directed evolution of a *Pseudomonas fluorescens* esterase demonstrated how error-prone PCR and mutator strains could systematically improve enzyme enantioselectivity — a contribution that has garnered over 100 citations and remains a foundational reference in the field. Complementing this, his development of a colorimetric assay for epoxide hydrolase activity (1999) provided researchers with practical tools for rapid enzyme characterization. His group later pioneered a fully automated robotic platform for high-throughput enzyme library screening (2016, 102 citations), dramatically accelerating the pace of protein engineering campaigns. Additional contributions include a polarimetric assay for detecting amino acid racemase activity, broadening the toolkit for enzyme discovery. Through combining molecular biology, automation, and analytical chemistry, Bornscheuer has consistently lowered barriers to enzyme optimization, leaving a lasting imprint on both academic research and biotechnology.
Research Focus
Key Achievements
Top Papers
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- 3A colorimetric assay suitable for screening epoxide hydrolase activity65 citations · 1999
- 4