Steven D. Flanagan
Papers
2
Total Citations
77
H-Index
2
About
Steven D. Flanagan is a molecular biologist whose pioneering work has centered on the development and refinement of ligation-mediated PCR (LM-PCR) for high-resolution genomic analysis. His primary research contributions lie in advancing quantitative in vivo footprinting and DNA damage and repair detection at the nucleotide level. Flanagan’s most influential paper, “Ligation-mediated PCR for quantitative in vivo footprinting” (2000), has garnered 68 citations, establishing a foundational method for mapping protein-DNA interactions and DNA lesions with exceptional sensitivity. In a subsequent study (2002), he further optimized LM-PCR parameters, including robotic liquid handling, to achieve the detection of break frequencies below one per kilobase in mammalian cells—a critical breakthrough for investigating in vivo DNA repair mechanisms. This work enabled researchers to quantify rare DNA damage events with unprecedented precision, directly impacting fields such as cancer biology and toxicology. Flanagan’s methodological innovations have been widely adopted, making him a key figure in the development of tools for studying genome integrity and transcriptional regulation.
Research Focus
Key Achievements
Top Papers
- 1Ligation-mediated PCR for quantitative in vivo footprinting68 citations · 2000
- 2Ligation-Mediated PCR: Robotic Liquid Handling for DNA Damage and Repair9 citations · 2002