Ken Tasaki
Papers
1
Total Citations
7
H-Index
1
About
Ken Tasaki is a researcher whose work sits at the intersection of synthetic biology, metabolic engineering, and renewable energy. His most cited study, from 2010, focuses on the production of *Candida antarctica* lipase B (CALB), a critical enzyme for biodiesel production. In this work, Tasaki pioneered an automated PCR gene assembly protocol on a robotic workcell to construct a synthetic CALB gene open reading frame. He then expressed this gene in an ethanologenic yeast, ingeniously fusing it with a lycotoxin-1 C3 variant to anchor the active enzyme to the yeast cell surface. This created a self-immobilized, resin-bound biocatalyst, significantly enhancing the efficiency and reusability of the enzyme for biodiesel synthesis. While his citation count of 7 reflects a niche but highly specialized contribution, the work demonstrates a sophisticated, multi-step approach to solving a real-world industrial challenge. Tasaki’s research exemplifies how automated synthetic biology can be leveraged to engineer robust, cost-effective biocatalysts for sustainable fuel production, a contribution that remains relevant for researchers in green chemistry and bioprocess engineering.
Research Focus
Key Achievements
Top Papers
- 1