A.J. Harker
Papers
2
Total Citations
78
H-Index
2
About
A.J. Harker is a bioanalytical chemist whose work has advanced the quantitative analysis of antiviral and therapeutic compounds in biological fluids. Their primary research focuses on developing robust, high-performance liquid chromatographic (HPLC) methods for drug quantification in human serum, with particular emphasis on antiretroviral and neuraminidase inhibitors. Harker’s most impactful contribution is a validated HPLC assay for 2′-deoxy-3′-thiacytidine (lamivudine), a cornerstone of HIV therapy, which has been cited 63 times for its reliability in clinical pharmacokinetic studies. This method enabled precise monitoring of drug levels, directly supporting dose optimization and safety assessments. Additionally, Harker pioneered an automated HPLC technique for the neuraminidase inhibitor GG167 (zanamivir), using pre-column fluorescence derivatization with benzoin to achieve high sensitivity—a notable innovation cited 15 times. This work demonstrated the feasibility of detecting low-concentration antiviral agents in complex matrices, aiding influenza treatment research. While Harker’s citation counts reflect focused, specialized contributions rather than broad recognition, their methods remain foundational for bioanalytical labs, underscoring a career dedicated to precision and reproducibility in therapeutic drug monitoring.
Research Focus
Key Achievements
Top Papers
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