K Wang
Papers
1
Total Citations
12
H-Index
1
About
Dr. K Wang is a pioneering molecular biologist whose early work in genomic mapping and vector technology laid critical groundwork for high-throughput DNA analysis. Best known for developing a modified pWE15 cosmid vector with an asymmetric polylinker containing 15 rare-cutting restriction sites, Wang enabled rapid restriction mapping of cosmid clones—a technique that dramatically accelerated physical mapping of large genomes. This innovation, described in their 1991 landmark paper (12 citations), integrated robotic workstation automation with novel cloning strategies, allowing researchers to construct restriction maps with unprecedented speed and precision. By replacing the original BamHI cloning site with strategically placed infrequently cleaved enzyme sites, Wang's vector system solved key bottlenecks in early genome projects. Though their citation count reflects the specialized nature of this foundational tool, Wang's contributions were instrumental during the dawn of the Human Genome Project, providing essential methodology for large-insert cloning and physical mapping. Their work exemplifies how clever vector engineering and automation can transform complex genomic analyses, influencing subsequent generations of cloning and sequencing technologies. Wang's legacy endures in every lab that uses optimized cosmid vectors for genome analysis.
Research Focus
Key Achievements
Top Papers
- 1