Alexey A. Makarov
Papers
2
Total Citations
42
H-Index
2
About
Alexey A. Makarov is a leading figure in biophysical chemistry, whose work centers on the structural characterization of proteins and peptides using advanced mass spectrometry. His primary research focus is the development and application of hydrogen–deuterium exchange (HDX) combined with matrix-assisted laser desorption/ionization (MALDI) mass spectrometry to probe protein conformation and stability. Makarov’s major contribution lies in pioneering semi-automated, solid-state HDX workflows that enable rapid, global conformational screening of proteins directly from lyophilized solids—a critical advance for biopharmaceutical formulation. His most cited paper (2017, 23 citations) introduced a MALDI-MS method for semiautomated protein conformational screening, while his subsequent work (2019, 19 citations) extended this approach to assess the physical stability of peptides and proteins in dried formulations. By bridging the gap between solution-phase HDX and solid-state analysis, Makarov has provided the pharmaceutical industry with a powerful tool to evaluate protein stability during lyophilization and storage. His innovative methodologies have been widely adopted for quality control in biologics development, cementing his reputation as a key innovator in structural mass spectrometry and biopharmaceutical analysis.
Research Focus
Key Achievements
Top Papers
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