Solid Phase Extraction and Analysis of THC and Metabolites from Whole Blood using a Novel Automated Procedure using Liquid Chromatography and Tandem Mass Spectrometry(LC-MS/MS)

Abstract

Solid Phase Extraction and Analysis of THC and Metabolites from Whole Blood using a Novel Automated Procedure using Liquid Chromatography and Tandem Mass Spectrometry (LC-MS/MS) In this study, solid phase extraction (SPE) is described using a novel automated procedure to isolate tetrahydrocannabininol and its metabolites from whole blood samples. SPE was performed on an automated system after samples of whole blood were precipitated with acetonitrile. The system was programmed to condition the SPE columns with methylene chloride, methanol,de-ionized (DI) water, and aqueous phosphate buffer (0.1 M pH 7). The samples were loaded by the robot after which the SPE columns were washed with DI water, aqueous phosphate buffer and dried also by the same unit.