Whole‐Genome Physical Mapping: An Overview on Methods for DNA Fingerprinting

Abstract

This chapter contains sections titled: Introduction Inception and Development of DNA Fingerprinting Clone-based Whole-genome Physical Mapping Source DNA Libraries for Whole-genome Physical Mapping Techniques and Methodologies Preparation of DNA from Bacteria-based Large-insert Clones Isolation of DNA from Bacteria-based Large-insert Clones Using the Clone-by-Clone Approach Isolation of DNA from Bacteria-based Large-insert Clones in 96-well Format Using a Manual Approach Isolation of DNA from Bacteria-based Large-insert Clones in 96-well Format Using the AutoGenprep 960 Robotic Workstation Fingerprinting the Clone DNA Using Restriction Enzymes Agarose Gel-based, Restriction Fingerprinting Method Polyacrylamide (Sequencing) Gel-based, Restriction Fingerprinting Method Automatic Sequencing Gel-and Capillary Electrophoresis-based Restriction Fingerprinting Methods Applications of DNA Fingerprinting for Genome Physical Mapping Whole-genome Physical Maps Generated from Large-insert Bacterial Clones by Fingerprint Analysis Comparison of Different Fingerprinting Methods Discussion Acknowledgements References