OTHER
The Automated Determination of NAD-Coupled Enzymes
Stanley Morgenstern, Richard V. Flor, Gerald Kessler, Bernard Klein
- Year
- 1966
- Citations
- 28
- Access
- Open access
Abstract
Abstract A precise automated procedure developed for determination of serum lactate dehydrogenase, using the Robot Chemist, measures absorbance of a cuprous-neo-cuproine complex formed by coupled reduction of the cupric-neocuproine reagent with enzymatically generated reduced nicotinamide adenine dinucleotide. Activity values obtained by this method, by the identical automated colorimetric method on the AutoAnalyzer, and by an automated spectrophotometric (34O-mµ) procedure show excellent agreement.
Keywords
AutoAnalyzerReagentNAD+ kinaseNicotinamide adenine dinucleotideChemistryAbsorbanceChromatographyLactate dehydrogenaseDehydrogenaseEnzyme
Related papers
OTHER
📊 26,957 cites
Statistical Learning Theory
Yuhai Wu, Vladimir Vapnik
1999
OTHER
Open access📊 20,501 cites
Fractional Differential Equations
Igor Podlubný
2025
OTHER
📊 18,993 cites
Applied Nonlinear Control
Jean-Jacques Slotine, Weiping Li
1991
OTHER
📊 13,277 cites
Genetic Programming: On the Programming of Computers by Means of Natural Selection
John R. Koza
1992