Identification of VCP/p97, Carboxyl Terminus of Hsp70-interacting Protein (CHIP), and Amphiphysin II Interaction Partners Using Membrane-based Human Proteome Arrays

Abstract

Proteins mediate their biological function through interactions with other proteins. Therefore, the systematic identification and characterization of protein-protein interactions have become a powerful proteomic strategy to understand protein function and comprehensive cellular regulatory networks. For the screening of valosin-containing protein, carboxyl terminus of Hsp70-interacting protein (CHIP), and amphiphysin II interaction partners, we utilized a membrane-based array technology that allows the identification of human protein-protein interactions with crude bacterial cell extracts. Many novel interaction pairs such valosin-containing amphiphysin and and to the interactions to that we the the and the with the of amphiphysin that human array technology the identification of protein-protein interactions that the of protein and the of Proteins mediate their biological function through interactions with other proteins. Therefore, the systematic identification and characterization of protein-protein interactions have become a powerful proteomic strategy to understand protein function and comprehensive cellular regulatory networks. For the screening of valosin-containing protein, carboxyl terminus of Hsp70-interacting protein (CHIP), and amphiphysin II interaction partners, we utilized a membrane-based array technology that allows the identification of human protein-protein interactions with crude bacterial cell extracts. Many novel interaction pairs such valosin-containing amphiphysin and and to the interactions to that we the the and the with the of amphiphysin that human array technology the identification of protein-protein interactions that the of protein and the of protein-protein interactions protein-protein carboxyl terminus of Hsp70-interacting valosin-containing protein protein-protein carboxyl terminus of Hsp70-interacting valosin-containing protein to biological their systematic identification a strategy the of cellular interaction a cellular protein interaction cell protein-protein interactions cell of and have to the and protein interaction of comprehensive to the protein of the of the protein-protein interaction of comprehensive of protein-protein interactions and human protein-protein interaction a the protein interaction of to a of the human protein-protein interaction protein the of a protein of the protein have a technology the identification of protein of protein other of protein of a technology and with the of with and to their of protein and the of novel and proteins. novel of protein of protein protein of the protein identification of protein the of protein and their systematic interaction and the to proteins. and For systematic screening protein that the of crude protein we the and of technology that allows the identification of with crude bacterial cell human proteins. novel the valosin-containing protein carboxyl terminus of Hsp70-interacting protein (CHIP), and amphiphysin II and with and the of protein the protein and screening of human For the of and amphiphysin II the and the and and and with the protein and the of protein and For a with the protein and the of protein For of interactions with the the a protein the and of to the of the and of protein the and protein of Proteins of human with of and and with the of protein of of and and and of of and and the cell a the to cell the a with of protein cell with with with and with and of with and to crude bacterial cell the protein to with and with of with with and to the a with of a of of a a the of the of the of the of of protein of and with bacterial protein of protein and with and with of Proteins and and of the and with a of the identification with with a protein and with and protein interaction of to For interaction the and the and the and to For of of the and and protein interaction of to and with the protein and the of protein For and with and and with with to and with to with with the of of a protein and of protein and of and and and a the tech