Robert M. Barkley
Papers
1
Total Citations
587
H-Index
1
About
Robert M. Barkley has made transformative contributions to the field of mass spectrometry imaging, particularly through his pioneering work on matrix application techniques. His most influential research, published in 2007, introduced sublimation as a method for applying organic matrices—such as 2,5-dihydroxybenzoic acid, sinapinic acid, and alpha-cyano-4-hydroxycinnamic acid—in MALDI imaging. This innovation, cited over 587 times, revolutionized sample preparation by enabling uniform, solvent-free matrix deposition, dramatically improving spatial resolution and reproducibility in molecular imaging. Barkley’s work has become a cornerstone for researchers studying tissue sections, allowing for precise mapping of proteins, lipids, and metabolites directly from biological samples. His contributions have not only advanced fundamental understanding of matrix behavior under reduced pressure and elevated temperature but also provided a practical, widely adopted tool that underpins countless studies in proteomics and metabolomics. Through this seminal achievement, Barkley has left an indelible mark on analytical chemistry, empowering scientists worldwide to explore the spatial distribution of molecules with unprecedented clarity.
Research Focus
Key Achievements
Top Papers
- 1Sublimation as a method of matrix application for mass spectrometric imaging587 citations · 2007