Oleg V. Krokhin

University of Manitoba, Research Manitoba

Papers

Total Citations

454

H-Index

About

Oleg V. Krokhin is a leading figure in proteomics and analytical chemistry, renowned for his pioneering work in peptide separation science and mass spectrometry. His most significant contribution is the development of an improved predictive model for retention times of tryptic peptides in ion-pair reversed-phase HPLC, published in 2004 and cited over 287 times. This model, built from 346 peptides across 17 protein digests, revolutionized the accuracy of liquid chromatography-mass spectrometry (LC-MS) workflows, enabling more reliable peptide identification and quantification in complex biological samples. During the 2003 SARS outbreak, Krokhin applied mass spectrometry to characterize proteins from the SARS virus, using convalescent sera to identify viral antigens—a critical early step in understanding the pathogen that earned over 167 citations. His work bridges computational prediction and experimental proteomics, directly impacting fields from biomarker discovery to systems biology. With a career marked by methodical innovation, Krokhin’s models and techniques remain foundational tools for researchers striving to unravel the proteome with precision and speed.