Janet Estee Kacharmina
Papers
2
Total Citations
248
H-Index
2
About
Janet Estee Kacharmina is a pioneering figure in single-cell molecular biology, whose work has fundamentally advanced our ability to analyze gene expression and protein content at the most granular level. Her primary research focuses on developing ultra-sensitive techniques for probing the molecular contents of individual cells and even subcellular regions. Her landmark 1999 paper, "Preparation of cDNA from single cells and subcellular regions," which has garnered 165 citations, established a foundational method for amplifying genetic material from minimal starting quantities, enabling researchers to explore cellular heterogeneity with unprecedented precision. Building on this, Kacharmina’s 2001 work on "Protein quantification from complex protein mixtures using a proteomics methodology with single-cell resolution" introduced the innovative immuno-detection amplified by T7 RNA polymerase (IDAT) technique. This method, with 83 citations, allows for the monitoring of proteins, lipids, and metabolites—and their modifications—at the single-cell level, overcoming a major bottleneck in proteomics. Her contributions have been instrumental in bridging genomics and proteomics, providing tools that empower researchers to dissect the molecular diversity of complex biological systems, from neural circuits to tumor microenvironments.
Research Focus
Key Achievements
Top Papers
- 1[1] Preparation of cDNA from single cells and subcellular regions165 citations · 1999
- 2