Douglas E. Brenneman
Papers
2
Total Citations
50
H-Index
2
About
Douglas E. Brenneman is a leading figure in functional genomics and high-throughput screening, with a career focused on developing and applying innovative technologies to accelerate drug discovery and target validation. His major contributions lie at the intersection of automation and molecular biology, where he pioneered robust, scalable methods for analyzing gene function and expression. His highly cited 2004 work on "High-Throughput siRNA-Based Functional Target Validation" (33 citations) established a cornerstone approach for systematically silencing genes to identify their biological roles, directly enabling more efficient target discovery. Complementing this, his work on "Gene Expression Analysis for High Throughput Screening Applications" (17 citations) introduced an automated high-throughput TaqMan RT-PCR method using the Allegro system, revolutionizing quantitative mRNA expression analysis by integrating liquid handling and sample tracking. This innovation allowed researchers to move beyond low-throughput assays, making large-scale gene expression profiling practical for screening campaigns. Brenneman’s achievements demonstrate a rare ability to bridge engineering and biology, creating tools that have become standard in the pharmaceutical and academic research communities. His work remains foundational for scientists seeking to understand gene function at scale.
Research Focus
Key Achievements
Top Papers
- 1High-Throughput siRNA-Based Functional Target Validation33 citations · 2004
- 2Gene Expression Analysis for High Throughput Screening Applications17 citations · 2004