Low-cost museum DNA extraction using magnetic beads v2

摘要

Modified from Korlevic et al 2021& Rohland et al 2018 for high throughput DNA extraction from historical specimens in natural history collections. Please cite these papers when using this method. An in depth SOP by Korlevic can be found here: https://dx.doi.org/10.17504/protocols.io.ewov1o3molr2/v1 Post lysis the protocol can be run by hand or on a magnetic bead based robot (e.g. Kingfisher Flex / Apex). v2 uses 5x lysate volume of binding buffer as tests have shown improved recovery over 10x volume. v2 replaces SeraSil beads with Qiagen MagAttract beads which provide a much cheaper option to G-Biosciences beads.