International Coordination of Large-Scale Human Induced Pluripotent Stem Cell Initiatives: Wellcome Trust and ISSCR Workshops White Paper

摘要

There is growing recognition of the potential value of human induced pluripotent stem cells (hiPSC) for understanding disease and identifying drugs targets. This has been reflected in the establishment of multiple large-scale hiPSC initiatives worldwide. Representatives of these met recently at a workshop supported by the Welcome Trust in the UK and in a focus session at the 2014 ISSCR annual meeting in Vancouver. The purpose was to discuss strategies for making thousands of hiPSC lines widely available with as few restrictions as possible while retaining financial viability and donor privacy. The outcome of these discussions is described here. There is growing recognition of the potential value of human induced pluripotent stem cells (hiPSC) for understanding disease and identifying drugs targets. This has been reflected in the establishment of multiple large-scale hiPSC initiatives worldwide. Representatives of these met recently at a workshop supported by the Welcome Trust in the UK and in a focus session at the 2014 ISSCR annual meeting in Vancouver. The purpose was to discuss strategies for making thousands of hiPSC lines widely available with as few restrictions as possible while retaining financial viability and donor privacy. The outcome of these discussions is described here. Main TextIntroductionThe ability to generate human induced pluripotent stem cells (hiPSCs) by direct reprogramming of somatic cells by simple overexpression of transcription factors initiated a paradigm shift in biomedical science (Takahashi et al., 2007Takahashi K. Tanabe K. Ohnuki M. Narita M. Ichisaka T. Tomoda K. Yamanaka S. Induction of pluripotent stem cells from adult human fibroblasts by defined factors.Cell. 2007; 131: 861-872Abstract Full Text Full Text PDF PubMed Scopus (14654) Google Scholar, Yu et al., 2007Yu J. Vodyanik M.A. Smuga-Otto K. Antosiewicz-Bourget J. Frane J.L. Tian S. Nie J. Jonsdottir G.A. Ruotti V. Stewart R. et al.Induced pluripotent stem cell lines derived from human somatic cells.Science. 2007; 318: 1917-1920Crossref PubMed Scopus (8045) Google Scholar). In just a few years, hiPSCs have opened new fields of investigation not only in cellular reprogramming itself but also in creating human models of inherited diseases for basic research in pathological mechanisms and drug target discovery. The increasing numbers of grant applications and publications that include hiPSCs (>7000 manuscripts have been published since 2007) are evidence of how these cells have captured the imagination of those both inside and outside this rapidly evolving large-scale initiatives have been to this growing and hiPSC for and and understanding the of disease in the of these large-scale initiatives to hiPSC lines from of and in and and the focus in applications in disease and drug these initiatives to to for with few that human disease models for are to include and and that are the are to with to to and of and and of donor hiPSC for for for disease and with lines from and and for research for and hiPSC lines from drug and for fibroblasts and for for research and by and by and for lines from and for disease target drug and pluripotent and of of hiPSC lines of and of the of hiPSC lines for diseases and in a new to of hiPSC lines from disease as as The of these as at the Trust and ISSCR to discuss the and with the of numbers of hiPSC is This the to for and hiPSC and The is that hiPSC lines in the a research of hiPSC the the available for was the to by large-scale hiPSC the of lines the are available to generate hiPSCs from somatic cells and are the transcription factors the in the of target that of the as this a et al., K. R. M. R. R. M. et fibroblasts to induced pluripotent stem PubMed Scopus Google Scholar). reprogramming is widely as that the are and et al., M. M. J. K. pluripotent stem cells PubMed Scopus Google et al., K. M. of human pluripotent stem cells a that not the PubMed Scopus Google et al., J. K. Smuga-Otto K. Tian S. Stewart R. in