The Krüppel-like Factor Epiprofin Is Expressed by Epithelium of Developing Teeth, Hair Follicles, and Limb Buds and Promotes Cell Proliferation

摘要

We identified a cDNA clone for epiprofin, which is preferentially expressed in teeth, by differential hybridization using DNA microarrays from an embryonic day 19.5 mouse molar cDNA library. Sequence analysis revealed that this cDNA encodes a member of the Krüppel-like factor family containing three characteristic C2H2-type zinc finger motifs. The full-length cDNA was obtained by the 5′ Cap capture method. Except for its 5′-terminal sequence, the epiprofin mRNA sequence is almost identical to the predicted sequence of Krüppel-like factor 14/Sp6 (specificity protein 6), which was previously identified in expressed sequence tag data bases and GenBank™ by an Sp1 zinc finger DNA-binding domain search (Scohy, S., Gabant, P., Van Reeth, T., Hertveldt, V., Dreze, Van P., and sequence is to in the of the of hybridization revealed that epiprofin mRNA is expressed by and in hybridization of epiprofin mRNA in of the in and the of an epiprofin revealed that this is in the and epiprofin is a and protein expressed by of teeth, and that in the of by We identified a cDNA clone for epiprofin, which is preferentially expressed in teeth, by differential hybridization using DNA microarrays from an embryonic day 19.5 mouse molar cDNA library. Sequence analysis revealed that this cDNA encodes a member of the Krüppel-like factor family containing three characteristic C2H2-type zinc finger motifs. The full-length cDNA was obtained by the 5′ Cap capture method. Except for its 5′-terminal sequence, the epiprofin mRNA sequence is almost identical to the predicted sequence of Krüppel-like factor 14/Sp6 (specificity protein 6), which was previously identified in expressed sequence tag data bases and GenBank™ by an Sp1 zinc finger DNA-binding domain search (Scohy, S., Gabant, P., Van Reeth, T., Hertveldt, V., Dreze, Van P., and sequence is to in the of the of hybridization revealed that epiprofin mRNA is expressed by and in hybridization of epiprofin mRNA in of the in and the of an epiprofin revealed that this is in the and epiprofin is a and protein expressed by of teeth, and that in the of by by and is a of this and a for the of molar is embryonic day embryonic day Krüppel-like embryonic day Krüppel-like the and the of this in the of the the which and the the the to the and and of of which and the the the and the the the the and which and the the is almost by and and family of in and in and this family a DNA-binding domain three C2H2-type zinc finger the and a domain the protein family by its sequence and an and of Sp1 and expressed and expressed in a and identified by a mouse expressed sequence tag data using the Sp1 zinc finger domain a and to by analysis Van Van protein a of and the sequence of the zinc finger domain is and in the domain is the factor an and to family to in and revealed and by the of the and of of the of the this a cDNA clone for epiprofin from a mouse molar cDNA by differential using DNA Sequence analysis revealed that epiprofin is by the and the sequence the previously predicted sequence for for a in the of epiprofin in that epiprofin and a in the of of mouse molar cDNA was and by differential hybridization using DNA and in a cDNA was in the using mRNA from of mouse cDNA from the molar cDNA using the and by The a and using a from mouse and was by using the and using in the of The DNA a of and and in a for preferentially to molar and of that epiprofin was in this The 5′ of the was obtained by the using molar the The was the for The full-length cDNA for epiprofin was obtained by using from mouse GenBank™ The 5′ of the for epiprofin the and was obtained by using mouse DNA and GenBank™ and protein in the and The sequence of epiprofin was the and The sequence for the full-length epiprofin and epiprofin containing the zinc finger domain the in and using the for was to and obtained a a The and obtained using and was from mouse using the of was for to which was a for cDNA was an for for for and for for and a for and of was by and to a