Phosphorylation of p53 on Key Serines Is Dispensable for Transcriptional Activation and Apoptosis

摘要

The p53 tumor suppressor is a key mediator of the cellular response to stress. Phosphorylation induced by multiple stress-activated kinases has been proposed to be essential for p53 stabilization, interaction with transcriptional co-activators, and activation of p53 target genes. However, genetic studies suggest that stress-activated phosphorylation may not be essential for p53 activation. We therefore investigated the role of p53 phosphorylation key and for p53 activation a We that not the phosphorylation of of the of and p53 induced by the and and and to p53 target and to We that p53 phosphorylation is not for activation of p53 target The p53 tumor suppressor is a key mediator of the cellular response to stress. Phosphorylation induced by multiple stress-activated kinases has been proposed to be essential for p53 stabilization, interaction with transcriptional co-activators, and activation of p53 target genes. However, genetic studies suggest that stress-activated phosphorylation may not be essential for p53 activation. We therefore investigated the role of p53 phosphorylation key and for p53 activation a We that not the phosphorylation of of the of and p53 induced by the and and and to p53 target and to We that p53 phosphorylation is not for activation of p53 target The tumor suppressor p53 is a that a of cellular to response to p53 the of of to that not p53 activation not for the by The is by a the p53 and tumor suppressor by transcriptional is for p53 and p53 that p53 and response to the interaction be to p53 to with for activation of target genes. p53 activation of p53 multiple by and and to that and to p53 phosphorylation has been and has been proposed to a role the and activation of the tumor studies been by the of that p53 and and and been to phosphorylation response to kinases been the to p53 phosphorylation the of and not the of p53 and been to a role the interaction the and phosphorylation the p53 and and studies to the that phosphorylation of p53 is a key for activation of the tumor suppressor response to cellular to proposed role of and of the p53 phosphorylation has been of However, of p53 phosphorylation is and of p53 is of for a tumor has been that phosphorylation the transcriptional of p53 of the of cellular response to of p53 a may be by to and interaction with of the transcriptional has been that and phosphorylation may the of p53 and phosphorylation has been to the interaction of p53 with transcriptional and phosphorylation of has been the activation of response has been to p53 and and transcriptional is phosphorylation of p53 suggest that p53 phosphorylation may role not of p53 of transcriptional the phosphorylation p53 been that and p53 not to studies with of suggest that not essential for p53 activation the of the and of the the p53 with and p53 to of p53 and activation of the p53 and and p53 by to not to p53 and not with of with of p53 that is of phosphorylation may for the role of p53 phosphorylation cellular that p53 induced by the is not key the of p53 to p53 target and p53 induced by the and to the that p53 is p53 phosphorylation is not for of p53 and and a been and the with and of to with for with of by of of a The the of p53 by the p53 by and and and by to and by the of and to by for p53 and and p53 to with and to the p53 and with p53 for to and and for to The and with a of to and the of to the The of the and by and a The of the and p53 to and with the for for that may and with and by for and the by the p53 and p53 a of and of interaction the p53 with and p53 with of p53 with to p53 stabilization, and activation of the p53 p53 by interaction with not the of to the of to p53 the the of p53 with the of p53 response to with the and We the and p53 and to by p53 and activation of the p53 the with a of of and the of for the and by a of the a of p53 and target and The the and the is to that of and p53 with the and by Phosphorylation that not p53 phosphorylation a response to is with the that and not the